Diazo Tablet Test

Diazo Tablet Test. Ictotest (diazo tablet test) Does detect lower limits than the pad (as low as 00501 mg/dL) 3 substances that do not have additional tests Urobilinogen Leukocyte Esterase Nitrite Four tests that ascorbic acid interferes with 1 Blood 2 Nitri.

The Ictotest tablets based on the diazotization reaction are utilized as a confirmatory assay for the presence of urinary bilirubin when a frontline test (ie urine strip or another semiquantitative method) has provided a positive bilirubin result The Ictotest reagent tablets can be used to ruleout the presence of interfering substances that may cause a false positive result.

Siemens Ictotest Reagent Tablets

A falsepositive reaction for urinary bile using the diazo tablet test may result after Mefenamic Acid administration If biliuria is suspected other diagnostic procedures such as the Harrison spot test should be performed Pregnancy Teratogenic Effects Pregnancy Category C PK Parameters Normal Healthy Adults (1845 yr)Value CV.

Physical and chemical examination of urine

A comparison has been made of the tablet test (“ictotest”) allowed to couplewitha diazo compound in tablet form to give acoloured complex It has beentried in America by Klatskin and Bungards (1953) Sobotka LuisadaOpper and Reiner (1953) Giorda Author Daphne Bryant F V FlynnCited by Publish Year 1955.

Urinalysis Reagent Strip Tests Principles, Interferences

Diazo reaction The sensitivity of the tests Many other compounds give a positive reaction with FeCL3 with different colors A tablet test is more sensitive than a dipstick Tablet test = 02 to 04 mg/dL Dipstick test = 005 to 01 mg/dL Falsenegative result Oxidation of the bilirubin in case of delayed testing Vitamin C (ascorbic acid).

Determination Of Pyridoxine By Means Of An Indicator Diazo Test Strip

IntroductionMaterials and MethodsResults and DiscussionConclusionsPyridoxine (vitamin B6) plays an important role in the body’s metabolism it is necessary for the normal functioning of the central and peripheral nervous system is a coenzyme of a number of enzymes acting on a nonoxidative exchange of amino acids participates in the exchange of tryptophan methionine cysteine glutamic and other amino acids and promotes the normalization of lipid metabolism There are known methods for the determination of pyridoxine in various preparations by means of chemical indicators – diazo compounds in combination with physicochemical methods of analysis Semiquantitative determination of pyridoxine in urine was carried out by preliminary separation of it from other substances by electrophoresis on paper with the appearance of spots on the electrophoregram with a diazotized streptocide with mercury dichloride to form complexes of azocompounds of redviolet color The drawback of the method was a large error The determination of pyridoxine in the Standard solutions of metal ions and standard buffer solutions were prepared by dissolution of weighed portions of chemically pure grade reagents in doubly distilled water Pyridoxine hydrochloride 35Dihydroxymethyl2methylpyridin3ol hydrochloride Vitamine B6 min 98 % aniline for synthesis 99 % Phenol for synthesis 99 % (Merck) were used “Cell” (see the scheme) epoxidized chromatographic paper (Whatman) based on cellulose containing 5% of the epoxy groups was used as a carrier of reagents for the preparation of test strips The above obtained cellulose carrier (Cell) was impregnated with 1% solution of 15bis(2amino5nitrophenyl)15dioxapentane in CHCl3 and was heated for 05 hours at 130°C The obtained reagent indicator paper (RIP) based on 2hydroxy4hydro4{2’[5’’(2amino5nitrophenyl)1’’5’’dioxapentyl]4’nitrophenyl}4azabutylcellulose was placed in a 1% HCl water solution and 02% NaNO2 water solution at 0−2°C during 20 minutes The surplus of r ТS RIPDiazo on a basis 2hydroxy4nitroso4{2’[5’’(2diazo5nitrophenyl)1’’5’’dioxapentyl]4’nitrophenyl}4azabutylcellulose of 4tolyl sulfate was synthesized by the method and entered into a reaction of a azocoupling with pyridoxine with the formation of RIPAzoPyridoxine – a tetradentate reagent that interacted with metal ions by the formation of complexes with a bathochromic shift silver – brown copper – blueviolet cobalt and zinc – dark brown color (Figure 1) The ocurrence of the azo group –N=N– in the pyridoxine molecule during the azocoupling process can pass only to the paraposition of the pуridine ring towards to the 1st type orientator OHgroup In the pyridyl group of the pyridoxine molecule there is only one paraposition active to the azocoupling towards to the hydroxy group all other sites are occupied by other substituents However the formed RIРAzoPyridoxine could be in the form of equilibrium isomers with tetradentate coordination groups o A sorptionspectrometric method for the determination of pyridoxine was developed by its template azo coupling with an indicator test strip based on 2hydroxy4nitroso4 {2 ‘ [5 “ (2diazo5nitrophenyl) 1” 5 “ dioxapentyl] 4’nitrophenyl} 4azabutylcellulose 4tolyl sulfate (RIPDiazo) in the presence of copper (II) the subsequent measurement of the reflection coefficient as function F of the test strip at 570 nm Author Vera M Ostrovskaya Alexander V Ivanov Dmitry Yu MarchenkoPublish Year 2019.